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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/01/2020 |
Actualizado : |
22/01/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PAZ, L.N.; HAMOND, C.; DIAS, C.S.; CURVELO, V.P.; MEDEIROS, M.A.; ORIÁ, A.P.; PINNA, M.H. |
Afiliación : |
LUCAS NOGUEIRA PAZ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLA SILVA DIAS, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; VITOR P. CURVELO, Getúlio Vargas Zoobotanic Park, Salvador, BA, Brazil; MARCO ALBERTO MEDEIROS, Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Ministry of Health, FIOCRUZ, Brazil; ARIANNE PONTES ORIÁ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; MELISSA HANZEN PINNA, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil. |
Título : |
Detection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution] |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
EcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0 |
ISSN : |
1612-9202 |
DOI : |
10.1007/s10393-019-01452-0 |
Idioma : |
Inglés |
Notas : |
Article history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019.
The online version of this article contains supplementary material, which is available to authorized users. |
Contenido : |
ABSTRACT.
Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers ≥ 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians.
© 2019, EcoHealth Alliance. |
Palabras claves : |
Crocodilian; Diagnosis; PLATAFORMA SALUD ANIMAL. |
Thesagro : |
LEPTOSPIROSIS; REPTILES; ZOONOSIS. |
Asunto categoría : |
A50 Investigación agraria |
Marc : |
LEADER 02457naa a2200301 a 4500 001 1060670 005 2020-01-22 008 2019 bl uuuu u00u1 u #d 022 $a1612-9202 024 7 $a10.1007/s10393-019-01452-0$2DOI 100 1 $aPAZ, L.N. 245 $aDetection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution]$h[electronic resource] 260 $c2019 500 $aArticle history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019. The online version of this article contains supplementary material, which is available to authorized users. 520 $aABSTRACT. Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers ≥ 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians. © 2019, EcoHealth Alliance. 650 $aLEPTOSPIROSIS 650 $aREPTILES 650 $aZOONOSIS 653 $aCrocodilian 653 $aDiagnosis 653 $aPLATAFORMA SALUD ANIMAL 700 1 $aHAMOND, C. 700 1 $aDIAS, C.S. 700 1 $aCURVELO, V.P. 700 1 $aMEDEIROS, M.A. 700 1 $aORIÁ, A.P. 700 1 $aPINNA, M.H. 773 $tEcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
07/06/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
CAPDEVIELLE, F.; BONNECARRERE, V.; ALONSO, M.; GARAYCOCHEA, S.; VALENZUELA, S. |
Afiliación : |
FABIAN MARCEL CAPDEVIELLE SOSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANA ALONSO, Tesista Facultad de Ciencias, UDELAR.; SILVIA GARAYCOCHEA, Tesista Facultad de Ciencias, UDELAR.; SEBASTIÁN VALENZUELA, Pasante Facultad de Ciencias. |
Título : |
Discriminación de genotipos y análisis de asociaciones entre marcadores moleculares y caracteres de interés agronómico. |
Complemento del título : |
Mejoramiento genético. III. Clasificación asistida por marcadores moleculares aplicada al Programa de Mejoramiento Genético de Arroz. |
Fecha de publicación : |
2004 |
Fuente / Imprenta : |
ln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2003-2004. Treinta y Tres (Uruguay): INIA, 2004. |
Páginas : |
cap. 5, p. 33-38. |
Serie : |
(INIA Serie Actividades de Difusión; 373) |
Idioma : |
Español |
Thesagro : |
ARROZ; FITOMEJORAMIENTO; GENOTIPOS; MARCADORES GENETICOS. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10088/1/Ad-373-cap.5-p.33-38.pdf
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Marc : |
LEADER 00772naa a2200229 a 4500 001 1030885 005 2018-06-07 008 2004 bl uuuu u00u1 u #d 100 1 $aCAPDEVIELLE, F. 245 $aDiscriminación de genotipos y análisis de asociaciones entre marcadores moleculares y caracteres de interés agronómico. 260 $c2004 300 $acap. 5, p. 33-38. 490 $a(INIA Serie Actividades de Difusión; 373) 650 $aARROZ 650 $aFITOMEJORAMIENTO 650 $aGENOTIPOS 650 $aMARCADORES GENETICOS 700 1 $aBONNECARRERE, V. 700 1 $aALONSO, M. 700 1 $aGARAYCOCHEA, S. 700 1 $aVALENZUELA, S. 773 $tln: INIA TREINTA Y TRES. Arroz: resultados experimentales 2003-2004. Treinta y Tres (Uruguay): INIA, 2004.
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